The deoxyribose assay: an assay both for ‘free’ hydroxyl radical and for site-specific hydroxyl radical production

Novel fluorometric assay for hydroxyl radical scavenging capacity (HOSC) estimation.

A novel fluorometric method was developed and validated for hydroxyl radical scavenging capacity (HOSC) estimation using fluorescein as the probe. A constant flux of pure hydroxyl radical is generated under physiological pH using a Fenton-like Fe3+/H2O2 reaction. The generation of pure hydroxyl radicals under the experimental conditions was evaluated and confirmed using electron spin resonance ...

Hydroxyl radical - Wikipedia, the free encyclopedia

Serum Hydroxyl Radical Scavenging Capacity as Quantified with Iron-Free Hydroxyl Radical Source

We have developed a simple ESR spin trapping based method for hydroxyl (OH) radical scavenging-capacity determination, using iron-free OH radical source. Instead of the widely used Fenton reaction, a short (typically 5 seconds) in situ UV-photolysis of a dilute hydrogen peroxide aqueous solution was employed to generate reproducible amounts of OH radicals. ESR spin trapping was applied to quant...

production of hydroxyl free radical, the main mechanism for removing steroid hormones by ultrasound

background: one of the new methods for elimination or destruction of estrogen hormones is ultrasound irradiation. the aim of this study was to determine the main mechanism of steroid hormones removal by ultrasound. methods: in this study, estrogen (e1) and 17 beta-estradiol (e2) were irradiated with ultrasound at different frequencies, powers, and exposure times in two cases: with and without b...

Estrogen diminishes postischemic hydroxyl radical production.

Reperfusion of blood flow to an ischemic myocardium is imperative to survival; ironically, it may also manifest several pathophysiological conditions. The most important of these are reperfusion arrhythmias and tissue injury and/or death. The mechanisms involved in reperfusion arrhythmias remain to be fully elucidated; however, increasing evidence indicates that reperfusion-induced arrhythmias ...